Coding

Part:BBa_K4769226:Design

Designed by: Echo Gilpin   Group: iGEM23_Cambridge   (2023-10-10)


cat (chloramphenicol resistance) promoter & coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 317
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 317
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 317
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 317
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: tgatcggcacgtaagaggt (+ desired overhangs) RV: cgagctcgatatcaaattacgc (+ desired overhangs)

References